CCM: Sequence Variation of Pi d3 for Rice Blast Resistance in Yunnan Rice Landrace 09-22-2016

The researchers analyzed the sequence variations of Pi d3 for rice blast resistance in 80 Yunnan rice landraces by resequencing of open reading frame (ORF).


The results show that there are 39 nucleotide variations in 2775 bp ORFs of 80 rice accessions with an average mutation rate of 1.41% compared with Pi-d3 Digu ORF. Based on nucleotide variations, The WTBX Pi-d3ORF of 80 rice accessions can be divided into 37 haplotypes. Among these materials, H8 (28.8%), H4 (11.3%) and H23 (5.0%) are high-frequency types while others belong to low frequency types, which shows that the WTBX Pi-d3 in Yunnan rice landraces is rich in genetic variations and haplotypes, but low in frequency.


There are 28 amino acids variations in 33 translation proteins of 37 haplotypes with a high frequency of pseudogene: 18 haplotypes with 32 rice landraces (include 12 indica rice varieties) are examined to have Pi-d3 pseudogenes, and the reason may be the permeation of Pi-d3 pseudogene to indica. Except for the published terminator at position 737, there is a new find that positions 32 and 467 of protein also have terminator. Pi-d3 shows differences in the type and frequency of haplotypes between the subspecies of indica and japonica, the ecotypes of lowland and upland rice, non-waxy and waxy rice which shows that there is a genetic differentiation in subspecies or ecotypes. The geographical distribution of 37 haplotypes has a characteristic of spreading over a large area while concentrating in small areas, and Puer, Banna and Lincang are the central areas with abundant haplotypes which means the types of haplotypes decreased outward progressively.


*News from a paper titled Sequence Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace delivered byYang Yi, Sun Yiding, Ma Jiqiong, Wang Yanyan and Xu Minghui from the Institute of Biotechnology and Genetic Resources/Key Laboratory of Biotechnology Research of Yunnan Province/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation of Ministry of Agriculture of Yunnan Academy of Agricultural Sciences.

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